Localization and trafficking of CXCR4 and CXCR7

Early stage researcher 11 (ESR11) project – Ali Isibilir
Supervision: Prof MJ Lohse, Dr D Calebiro, Dr T Sungkaworn, Prof C Hoffmann
Host: UWUE (DE) – University of Würzburg, Institute of Pharmacology and Toxicology and Rudolf Virchow Center

I- Project proposal:

1. Determine localization and trafficking of CXCR4 and CXCR7 in response to chemokines using high-resolution microscopy.
2. Determine the effect of CXCR4 and CXCR7 heteromerization on localization and trafficking
3. Evaluate the effect of phosphorylation barcoding and intracellular partners on CXCR4, CXCR7, and heteromer localization and trafficking.

We have pioneered single molecule microscopy in the analysis of GPCRs using fluorescently labeled receptors and total internal reflection fluorescence (TIRF) microscopy. With these methods, we can actually see receptors how they move at the cell surface and how they interact with each other and with other proteins. Analysis of receptor movements can then be done by single particle tracking. This allows the determination of mobility, subcellular localization as well as aggregation and transient interactions of cell surface proteins. In this project, we will apply these technologies to chemokine receptors in order to investigate their behaviour and interactions at the cell surface, and to study how regulatory mechanisms influence the receptors’ behavior.
Planned secondments: CNRS (FR), UNOTT (UK).


II- Requirement candidate:

Required diploma: MSc degree in life or natural sciences (e.g. biophysics, biochemistry, biology, pharmacy)
Required expertise: Molecular biology, cell culture, microscopy
Recommended expertise: Fluorescence microscopy, basic biophysics courses, interest in mathematics and basic programming

Key publications:
1. Lohse MJ, Nuber S, Hofmann C (2012) Fluorescence/bioluminescence resonance energy transfer techniques to study G-protein-coupled receptor activation and signaling. Pharmacol Rev. 64, 299–336.

2. Emami-Nemini A, Roux T, Leblay M, Bourrier E, Lamarque L, Trinquet E, Lohse MJ (2013) Time-resolved fluorescence ligand binding for G-protein-coupled receptors. Nature Prot. 8, 1307-1320.

3. Calebiro D, Rieken F, Wagner J, Sungkaworn T, Zabel U, Borzi A, Cocucci E, Zürn A, Lohse MJ (2013) Single-molecule analysis of fluorescently labeled GPCRs reveals receptor-specific complexes with distinct dynamics and organization. Proc. Natl. Acad. Sci. USA 110, 743–748.

4. Sungkaworn T, Rieken F, Lohse MJ, Calebiro D (2014) High-resolution spatiotemporal analysis of receptor dynamics by single-molecule fluorescence microscopy. J. Vis. Exp. 2014, 89.

Follow us on

Great way to finish the week off with some #FCS measurements of CXCR4-expressing HEK cells at @UoNLifeSci 🔬 #FluorescenceFriday

Hello everyone!
I am Noemi Karsai and I’m the next in our #ESR introduction series. I’m #ESR11 and I’m originally from Hungary, currently doing my PhD at @UoNLifeSci @COMPARE_UoBUoN, UK.

The transatlantic ECI GPCR symposium #ECIGPCR is about to start, we are ready!! Thanks to the organizers for their excellent job gathering almost 500 people across the globe! @cyclic_Andreas @NicoleAPerry1 @BenderSci @DesislavaNeshe1

Hello 🙂
I am here to continue the series in which all #ESRs are presenting themselves. I am Viviana Marolda and I am in my first year of PhD. I am #ESR13, originally from Italy, and currently, I am working as a PhD student in @CBMSO_CSIC_UAM, at Universidad Autonoma de Madrid.

Hey twitter!
I am @DehanComez. I am taking over the Oncornet account for a while to start a series of all #ESRs writing about themselves and their projects. I am #ESR5, originally from Turkey and right now I am working as a PhD student in @COMPARE_UoBUoN , @UniofNottingham , UK.

New review on #gpcr structural dynamics out in COSB. Great teamwork with the @JanaSelent group. Thanks to @Lundbeckfonden and @novonordiskfond https://lnkd.in/eyjrA5V

ONCORNET2.0 is the successor to #ONCORNET. You can see some of the work of ESRs from the first ONCORNET in this special issue of @MolPharmJournal from 2019, with reviews on #CXCR4 and #ACKR3 structure and function: https://molpharm.aspetjournals.org/content/96/6

Hi everyone – we’re on Twitter! ONCORNET2.0 is a #MarieCurie ITN of 16 ESRs across Europe studying #chemokine #GPCRs #CXCR4 and #ACKR3 in cancer. Our projects cover molecular dynamics, medchem, #pharmacology through to translational work. Follow us for updates from our ESRs!

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Contact details

Please contact us at:

ONCORNET Coordinator
VU University Amsterdam
The Netherlands