Functional proteomics of CXCR4 and CXCR7-associated signalling networks

Early stage researcher 12 (ESR12) project – Amos Fumagalli
Supervision: Dr P Marin, Dr M Séveno, Dr S Chaumont-Dubel, Dr S Urbach
Host: CNRS (FR) – Institut de Génomique Fonctionnelle, CNRS UMR 5203, INSERM U1191,

Université de Montpellier

I- Project proposal:

1. Identify intracellular partners (GIPs) of CXCR4 and CXCR7 using an AP-MS proteomics strategy.

2. Determine functional consequences of CXCR4 and CXCR7 association with identified GIPs.

3. Decipher the phosphoproteomes resulting from CXCR4 and CXCR7 activation by different modulators.

Proteins interacting with epitope-tagged versions of CXCR4 and CXCR7 receptors will be purified by immunoprecipitation and affinity-purified proteins will be systematically identified by Nano-flow liquid chromatography coupled with Fourier transform tandem mass spectrometry (Nano-LC-FT-MS/MS). The influence of identified partners on receptor-operated signal transduction will be investigated by overexpressing or silencing expression of candidate partners and using tools and/or biosensors developed in this consortium. Phosphoproteomes generated upon receptor activation by different modulators will be deciphered by combining Stable Isotope Labelling by Amino acids in Cultured cells (SILAC) and a double-phosphopeptide enrichment procedure consisting of Hydrophilic Interaction Liquid Chromatography (HILIC) followed by Immobilized Metal Affinity Chromatography (IMAC). Planned secondments: Actelion (CH), Vivia Biosystems (ES)


II- Requirement candidate:

Required diploma: MSc degree in molecular/biomedical Life Sciences, Pharmaceutical Sciences or related Life Science degree.

Required expertise: cell culture, cell-based assays, biochemistry, molecular biology. Recommended expertise: Basic knowledge in mass spectrometry, proteomics, protein databases, bioinformatics and statistics.

Key publications:
1. Duhr, F., Deleris, P., Raynaud, F., Seveno, M., Morisset-Lopez, S., Mannoury la Cour, C., Millan, M. J., Bockaert, J., Marin, P. & Chaumont-Dubel, S. (2014) Cdk5 induces constitutive activation of 5-HT6 receptors to promote neurite growth Nat Chem Biol 10, 590-597. News and Views: Seo and Tsai. Neuronal differentiation: 5-HT6R can do it alone. Nat Chem Biol 10, 590-597.

2. Karaki, S., Becamel, C., Murat, S., Mannoury la Cour, C., Millan, M. J., Prezeau, L., Bockaert, J., Marin, P. & Vandermoere, F. (2014) Quantitative phosphoproteomics unravels biased phosphorylation of serotonin 2A receptor at Ser280 by hallucinogenic versus nonhallucinogenic agonists. Mol Cell Proteomics 13, 1273-1285.

3. Meffre, J., Chaumont-Dubel, S., Mannoury la Cour, C., Loiseau, F., Watson, D. J., Dekeyne, A., Seveno, M., Rivet, J. M., Gaven, F., Deleris, P., Herve, D., Fone, K. C., Bockaert, J., Millan, M. J. & Marin, P. (2012) 5-HT(6) receptor recruitment of mTOR as a mechanism for perturbed cognition in schizophrenia. EMBO Mol Med 4, 1043-1056.

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Great way to finish the week off with some #FCS measurements of CXCR4-expressing HEK cells at @UoNLifeSci 🔬 #FluorescenceFriday

Hello everyone!
I am Noemi Karsai and I’m the next in our #ESR introduction series. I’m #ESR11 and I’m originally from Hungary, currently doing my PhD at @UoNLifeSci @COMPARE_UoBUoN, UK.

The transatlantic ECI GPCR symposium #ECIGPCR is about to start, we are ready!! Thanks to the organizers for their excellent job gathering almost 500 people across the globe! @cyclic_Andreas @NicoleAPerry1 @BenderSci @DesislavaNeshe1

Hello 🙂
I am here to continue the series in which all #ESRs are presenting themselves. I am Viviana Marolda and I am in my first year of PhD. I am #ESR13, originally from Italy, and currently, I am working as a PhD student in @CBMSO_CSIC_UAM, at Universidad Autonoma de Madrid.

Hey twitter!
I am @DehanComez. I am taking over the Oncornet account for a while to start a series of all #ESRs writing about themselves and their projects. I am #ESR5, originally from Turkey and right now I am working as a PhD student in @COMPARE_UoBUoN , @UniofNottingham , UK.

New review on #gpcr structural dynamics out in COSB. Great teamwork with the @JanaSelent group. Thanks to @Lundbeckfonden and @novonordiskfond

ONCORNET2.0 is the successor to #ONCORNET. You can see some of the work of ESRs from the first ONCORNET in this special issue of @MolPharmJournal from 2019, with reviews on #CXCR4 and #ACKR3 structure and function:

Hi everyone – we’re on Twitter! ONCORNET2.0 is a #MarieCurie ITN of 16 ESRs across Europe studying #chemokine #GPCRs #CXCR4 and #ACKR3 in cancer. Our projects cover molecular dynamics, medchem, #pharmacology through to translational work. Follow us for updates from our ESRs!

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Contact details

Please contact us at:

ONCORNET Coordinator
VU University Amsterdam
The Netherlands