Pharmacological analysis of photoactivatable CXCR4/ACKR3 ligands in vitro/in vivo
Early stage researcher 7 (ESR7) project
Supervision: Prof. Dr. Rob Leurs, Dr. Henry Vischer, Dr. Maikel Wijtmans
I- Project proposal:
The project aims for the development of photopharmacological approaches for the CXCR4 and ACKR3 chemokine receptors. New assays to dynamically modulate these chemokine receptor systems with light-switchable ligands will be developed. Main focus will be on in vitro, cell-based assays, but ultimately light-induced in vivo modulation of CXCR4 and/or ACKR3 function is the final aim within this project.
This project will entail the assay development of new photopharmacology assays to evaluate photoswitchable small-molecule CXCR4 and ACKR3 ligands (agonists/antagonists). The characterization of new photoswitchable ligands can be supported by computer-aided drug design and GPCR mutation studies where necessary. Design and synthesis of photoswitchable ligands will be conducted by fellow ESR1.
InterAx (Switserland) - candidate will get exposed to relevant dynamic cell-based assays.
INSERM (Paris, France) - candidate will work on biological characterization of photoswitchables compounds in relevant models of disease.
II - Requirement candidate:
Required diploma: A MSc degree in Biochemistry or Pharmacology or related Biomedical degrees
Required expertise: The candidate has a strong background (theoretical and practical) in cell biology, signal transduction and preferably pharmacology and/or molecular biology.
Recommended expertise: Expertise in GPCR molecular and cellular pharmacology is a strong pre. Previous experience with biosensor-based assay development and/or photopharmacology is also highly valued
- Hauwert et al., J. Am. Chem. Soc. 2018, 140, 4232
- Hauwert et al., Angew. Chem. Intl. Ed. 2019, 58, 4531
- Gómez-Santacana et al., Angew. Chem. Intl. Ed. 2018, 57, 11608
- Gómez-Santacana et al., Beilstein Arch. 2019, doi:10.3762/bxiv.2019.69.v1
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